Figure 2

Protein categories of biological processes and cellular components affected by DM. Biological processes (a) and cellular components (b) decreased (a1,b3) or increased (a2,b4) in abundance in DM samples relative to NDM analyzed by PIANO (10% FDR). X-axis represents the number of proteins in each category; y-axis represents the value for a given category analyzed by enrichment test-statistic and related to mean of t-statistics for individual in-category proteins. Categories in lower right quadrants have multiple proteins with small abundance differences between DM and NDM groups, while categories in upper left quadrants have fewer proteins with larger abundance differences between DM and NDM groups. Categories displayed are a subset of all affected categories selected to minimize redundancy. (c) Differentially regulated pathways: Volcano plots of protein abundance for categories affected by DM. Protein abundance in categories related to TCA cycle, fatty acid metabolism, mitochondria, and cellular respiration were decreased, and immunity and inflammation proteins were increased in DM (PIANO, 10% FDR). Light grey points indicate proteins that are category members, triangles indicate proteins significantly regulated between DM and NDM (limma, q < 0.1).