Figure 2 | Scientific Reports

Figure 2

From: Cystin genetic variants cause autosomal recessive polycystic kidney disease associated with altered Myc expression

Figure 2

Cystin-GFP fusion protein expression in renal collecting duct. Immunohistochemical detection of cystin-GFP fusion protein (green, A–C), AQP2 (red, D–F) and merged (G–I) in kidney tissues from 6-week-old mice of the indicated genotypes. The Rosa26-Cys1-GFP/+ mice (A, D, G) do not express cystin-GFP fusion protein due to the absence of the Ksp-Cre transgene. The Rosa26-Cys1-GFP/+; Ksp-Cre/+ mice (B, E, H) have a wild-type Cys1 gene and express cystin-GFP fusion protein in Cre-positive cells. The cpk/cpk; Rosa26-Cus1-GFP/+; Ksp-Cre/+ rescue mice (C, F, I) express cystin-GFP fusion protein in Cre-positive cells. Cell nuclei are stained with DAPI (blue). Scale bars equal 50 µm. Images are representative of tissue sections from 3 animals.

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