Figure 1 | Scientific Reports

Figure 1

From: CRISPR/Cas9 small promoter deletion in H19 lncRNA is associated with altered cell morphology and proliferation

Figure 1

(A) Schematic representation of murine H19DMR and H19 promoter sequence (NC_000073.3 Chromosome 7 F5). The H19DMR is an imprinting control region that regulates H19 and IGF2 gene activity in an enhancer competition and insulator binding model. The differentially methylated region (DMR) near to H19 promoter is unmethylated in the maternal allele, which allows the attachment of the CTCF insulator protein and H19 expression. The insulator activity modifies the chromatin and prevents the enhancer activation of the IGF2, which is silenced. On the paternal allele, the methylation blocks the insulator biding, whereas makes conformational modifications in the H19 promoter, which is silenced on the paternal allele, while the enhancer acts on the IGF2 gene that is paternally expressed. (B) H19 predicted promoter region sequence (GRCm38/mm10 Chr7:142577859-142579404). The guide RNAs (gRNAs) (green) were designed to flank the promoter region, including 1301 base of pairs (bp) upstream and 225 bp downstream to the start codon “ATG” (blue arrow) of the first exon. 14 TATA Box sequences (highlighted in grey) were identified.

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