Figure 4
EZH2 regulates DUSP-1 and DUSP-6 expression through miR200a–c. MiR-200a (a), miR-200b (b), miR-200c (c), miR-141 (d), and miR-429 (e) expression levels were determined by qPCR in HUVEC exposed to FSS (20 dyne/cm2), transduced HUVEC with scr or shEZH2 and normalized to static control cells. H3K27me3 enrichment around the transcription start site (TSS) of the miR-200b/a/429 cluster was determined using ChIP in shEZH2-HUVEC and scr-HUVEC (f) and MEK5D-HUVEC and EV-HUVEC (g). H3K27me3 enrichment is shown as area under the curve (AUC) compared to input samples (h). H3K27me3 enrichment at the around the TSS of miR-200c/141 cluster was determined using ChIP in scr-HUVEC and shEZH2 (i) and in MEK5D-HUVEC and EV-HUVEC (j). H3K27me3 enrichment is shown as AUC compared to input samples (k). Data is expressed as mean ± S.D. of all individual observations. Gene expression data were obtained from 4 independent experiments. Data from ChIP experiments were obtained from three independent experiments. All data was analyzed by ANOVA followed by Bonferroni post hoc tests. *P < 0.05, **P < 0.01, ***P < 0.001.
