Figure 5 | Scientific Reports

Figure 5

From: Reciprocal regulation of endothelial–mesenchymal transition by MAPK7 and EZH2 in intimal hyperplasia and coronary artery disease

Figure 5

miRNA-200 family members regulated DUSP-1 and DUSP-6 expression. Luciferase reporter assays for microRNA binding were performed for the 3’UTR of DUSP-1 in COS7 cells with ectopic expression of miR-200a, -200b, -200c, -141, -429 or SCR (a). DUSP-1 expression levels were determined by qPCR in HUVEC with ectopic expression of miR-200b, miR-200a, miR-429, miR-200c, miR-141 or scr, normalized to control. All miRNA-200 family members decrease DUSP-1 expression in HUVEC (b). Luciferase reporter assay identified miR-200a and miR-141 to target DUSP-6 (c) and ectopic expression of miR-200a or miR-141 decreases DUSP-6 expression in HUVEC (d). Data is expressed as mean ± S.D. of all individual observations. Gene expression data and Luciferase activity measurements were obtained from 4 independent experiments. All data was analyzed by ANOVA followed by Bonferroni post hoc tests. *P < 0.05, **P < 0.01, ***P < 0.001.

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