Figure 7

Ectopic expression of miRNA-101, miRNA-141 and miRNA-200a inhibits endothelial dysfunction and EndMT. MAPK7 (a) and EZH2 (b) protein expressions were determined using western blotting, in HUVEC treated with 10 ng/ml TGFb1 with ectopic expression of miRs-101, -200a and -141 and normalized to untreated control cells. The expression of VE-Cadherin (c,d) and SM22a (c,e) were assessed by immunofluorescence and quantified using TissueFaxs analyses. Endothelial cell permeability was assessed using transwell FITC-dextran leakage (f) and collagen gel contraction (g) was assessed as a mesenchymal cell function. The angiogenic sprouting behavior of endothelial cells was assessed using the Matrigel assay (h). Data is expressed as mean ± S.D. of all individual observations. Cell culture data was obtained from 4 independent experiments. Data from multiple groups were analyzed by ANOVA followed by Bonferroni post hoc tests. *p < 0.05, **p < 0.01, ***p < 0.001. Scale bar = 20 µm.