Table 3 Primers used for CHIP-qPCR assays.
Genomic region | Chr | Sense sequence | Antisense sequence |
|---|---|---|---|
miR-200b/a/429 | |||
−2.5 kb | 1 | GGAGGAGCTGGTGTGTTCTC | CAAAGCCGCCATTTCACC |
−2.0 kb | 1 | GCGGTGATGATTAACCCAAC | GTGGCCACAGGTCAAGAAAT |
−1.5 kb | 1 | GGTGAGAACGCAATGACTGA | CTCCCACTGCCAGGTTCA |
−1.0 kb | 1 | TTGGAGGAGGAGACTGGAAC | AGTTTTCTGGCACCTTCCAC |
−0.5 kb | 1 | GACCAGCAGACACACAAACC | GACCCCTCTCCCATGCTG |
TSS | 1 | TACTGAGCTTCCCAGCGAGT | AATGCTGCCCAGTAAGATGG |
+ 0.5 kb | 1 | GAGCAGGACCCAACAGAGG | CAGGAGGGAAGATGGCTGT |
miR-200c/141 | |||
−2.5 kb | 12 | CACCTCCAGGTTCCAGCTAC | AAATGCTTCCACAGGGTCAG |
−2.0 kb | 12 | ACAGGGTGGTTGTGAAAAGC | CGCCACGGTAAAATGAGAAT |
−1.5 kb | 12 | CATGTCAGGAGTGGGGTTTC | CTTTGGGACCTGTGCTGTCT |
−1.0 kb | 12 | GACTCCACTGAGGGCTGTG | TGAAGTTACCCACCGCTACC |
−0.5 kb | 12 | GCCTAGAGGAGTGGCCAAG | GGTGTGTCCTCCTGCCATAG |
TSS | 12 | AGGGCTCACCAGGAAGTGT | AGGATCCCTGCGGAAAAG |
+ 0.5 kb | 12 | CCCTGTAGCAACTGGTGAGC | GGGAGCCATCTTTACCAGAC |
miR-101-1 | |||
−2.5 kb | 1 | CCACAGGCCTGGTTGTAGAT | AATCATTGGCCTTGGTGAAG |
−2.0 kb | 1 | TGGGTAGAGCAGAGGGAAGA | ATCCTTCATTGTGCCAGTCC |
−1.5 kb | 1 | TGACCGCAGACTGAGACTCTT | GCCAGGGAGAGAAAAACCAT |
−1.0 kb | 1 | TGGAGGTTGAAGATGTGTGC | CTGGTCTTGACCTCCTGAGC |
−0.5 kb | 1 | TGACAGCAGCAGCAATAACA | CCAGCTTACTGAAGTGAAGAAAGA |
TSS | 1 | TTCTTCCTGGGTACGGTGAG | CCGACACAGTGACTGACAGG |
+ 0.5 kb | 1 | ACTGACTGTGCCTCCCTGAC | TGAGCACTTTGAAGACAGGA |
DUSP-1 (MKP-1) | |||
−2.5 kb | 5 | CCTCACCCCTGCTCTTTATG | ATGGCTTGCAGTGACCTTCT |
−2.0 kb | 5 | CAGCAAGGGAGGAGAGAGAA | GCCTGGTGACAGAGCAAGAC |
−1.5 kb | 5 | GCCTCGCTTAGCTTGTGTGT | CACTCCATGCCCTGAACTTT |
−1.0 kb | 5 | GCAGTGGATTCCAGGGTTT | GAAAGGGATGGAGAAGCTCA |
−0.5 kb | 5 | GCTTCCTGTGCTTTTGCATAC | CCCCAGTAGTGTGGTTCTGG |
TSS | 5 | CGCTTTTGGACTGAGAGAGG | CTCGCTGCGAAGGACATT |
+ 0.5 kb | 5 | AGGGCGTACCTTTGAGGAAG | GTGGTGTTGCTGGACGAG |
DUSP-6 (MKP-3) | |||
−2.5 kb | 12 | AGGCCTAGGTTGCCAATTTT | AAAATGGTGCGGAGAGGAG |
−2.0 kb | 12 | ATTGGAAGCCGGATGGAG | GCAGGCTTCGGCACTTTTAT |
−1.5 kb | 12 | AATGATTTCTGGGCAAGGAG | GGTCTTGCGGGAGGACTT |
−1.0 kb | 12 | GACCCAAGTTCGCCTTAACC | ACACAGCCTCGGCTAAAAGA |
−0.5 kb | 12 | AGGCAGCTCCTCAATGGATA | TCATCAACACAACCTGTTCCA |
TSS | 12 | GTCTTGCTGATCGCCATTTC | AGCTCGACCCCCATGATAG |
+ 0.5 kb | 12 | GGAAGCGAGTGGATTCTGAG | CGCGTGGATTGAAAATACCT |
