Figure 5
From: Analysis of the signal cross talk via CCL26 in the tumor microenvironment in osteosarcoma
Changes in expression of Src and its downstream factors related to invasive potential. (A) Changes in phosphorylation and the expression of protein factors relating to invasive potential were analyzed. Decreased phosphorylation of Src, FAK, MEK and ERK in MG63 cells was noted in the group administered anti-CCL26 Ab. (B) The quantification of western blot analysis. (C) Immunofluorescence staining of cultured MG63 cells showed decreased CCL26 and p-Src in the group administered anti-CCL26 Ab. Original magnification, × 400; Scale bars: 50 μm. (D) The number of Rac and p-Src positive cells per unit area. Data represents represent the mean ± SD of three independent experiments. p < 0.05 was considered to indicate significance: (*) p < 0.05, (**) p < 0.01.
