Figure 3

Representative images of islet immunostaining of an NGT female in her 50 s photographed under a laser scanning confocal microscope. (A) Triple immunostaining of GCG (white), NKX6.1 (green), and ARX (red). White arrowhead shows ARX-negative NKX6.1⁺/GCG⁺ cell. (B) Triple immunostaining of GCG (white), NKX6.1 (green), and ARX (red). White arrowhead shows ARX-positive NKX6.1⁺/GCG⁺ cell. (C) Triple immunostaining of GCG (green), NKX6.1 (white), and INS (red). White arrowhead shows INS-negative NKX6.1⁺/GCG⁺ cell. (D) Triple immunostaining of GCG (green), NKX6.1 (white), and INS (red). White arrowhead shows INS-positive NKX6.1⁺/GCG⁺ cell. (E) Triple immunostaining of GCG (green), ARX (white), and INS (red). White arrowhead shows INS-negative ARX⁻/GCG⁺ cell. (F) Triple immunostaining of GCG (green), ARX (white), and INS (red). White arrowhead shows INS-positive ARX⁻/GCG⁺ cell. (G) Triple immunostaining of GCG (red), ARX (white), and SST (green). White arrowhead shows SST-negative ARX⁻/GCG⁺ cell. (H) Triple immunostaining of GCG (red), ARX (white), and PP (green). White arrowhead shows PP-negative ARX⁻/GCG⁺ cell. GCG glucagon, NKX6.1 NK homeobox 6.1, INS insulin, ARX aristaless-related homeobox, SST somatostatin, PP pancreatic polypeptide, DAPI 4′,6-diamidino-2-phenylindole. Bars = 50 µm.