Figure 6
Effect of S. aureus clinical strains (initial and relapse) on the early differentiation of OBß1 cells. (A) Quantification of internalization and intracellular survival of S. aureus 8325–4 and 8325–4∆fnbAB into OBß1 osteoblasts. (B) Effect of S. aureus 8325–4, 8325-4heat killed and 8325–4∆fnbAB on ALP activity. Results are expressed as a proportion of the activity detected for the non-infected condition. (C) Effect of S. aureus 8325–4, 8325-4heat killed and 8325–4∆fnbAB on the expressions of ALP, RUNX2, COL1A1, expressed as a fold change compared to the non-infected condition. At least 2 independent experiments in technical duplicates were performed. * and ** mean P < 0.05 and P < 0.01 respectively.
