Figure 5 | Scientific Reports

Figure 5

From: Distinct calcium regulation of TRPM7 mechanosensitive channels at plasma membrane microdomains visualized by FRET-based single cell imaging

Figure 5

Time-lapse fluorescence resonance energy transfer (FRET) ratio images of the Michigan Cancer Foundation-7 cells in false colors. Naltriben was added 5 min after the beginning of the experiment. The colored scale bar represents the range of the biosensor FRET/enhanced cyan fluorescent protein (CFP) emission ratios. The warmer the color of the pixel, the higher the Ca2+ concentration reported. Chemical pretreatment or RhoA mutant vector co-transfection lowered TRPM7-induced Ca2+ influx, hence, diminished the FRET/CFP ratio signal change. CytoD, cytochalasin-D. Scale bar = 20 μm.

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