Figure 7
Line graphs showing that (a) PF-573228 and (b) PP-1 chemical pretreatment of MCF-7 cells decreased TRPM7-induced Ca2+ ion influx. The bar chart with the values of fluorescence resonance energy transfer (FRET)/Enhanced cyan fluorescent protein (CFP) ratio change 12 min after the beginning of the experiment; (c) from (a) and (d) from (b). Student’s t test, ****P < 0.0001. NTB, naltriben; n, number of cells. Error bars in the line plots represent the standard error of the mean (P < 0.05). (e) Time-lapse FRET ratio images of the MCF-7 in false colors. The colored scale bar represents the range of the biosensor FRET/CFP emission ratios. The warmer the color of the pixel, the higher the Ca2+ concentration reported. Scale bar = 20 μm.
