Figure 4

Age-dependent increase of [³H]CHDI-180 binding in HOM zQ175 HD mouse brains. (A) Radiometric filter trap assay (RAFTA) showing an age-dependent increase in [³H]CHDI-180 binding to aggregates in brain homogenates of HOM zQ175 HD mice. (B) Example images of [³H]CHDI-180 binding in RAFTA. (C) In vitro ARG showing an age-dependent increase in [³H]CHDI-180 binding to brain sections of HOM zQ175 HD mice. Specific binding (SB) of 0.5 nM [³H]CHDI-180 in striatum (STR), cortex (CTX) and hippocampus (HPC) of 3-, 6-, 9- and 12-month-old HOM Q175 mice and age-matched WT mice. (D) Example images of [³H]CHDI-180 binding. Data are depicted as mean ± SD from a total of n = 3 brain hemispheres per age and genotype.