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Figure 1

From: Competitive ELISA for a serologic test to detect dengue serotype-specific anti-NS1 IgGs using high-affinity UB-DNA aptamers

Figure 1

Dengue diagnostics using ELISA formats. (a) Schematic illustration of general detection sensitivity patterns for dengue viral (DENV) NS1 protein, virus (genome), and DENV-reactive IgM and IgG, in primary and secondary DENV infections8,35. (b) Direct DEN-NS1 detection in patient blood, by using our UB-DNA aptamers specific to each serotype NS1 protein as capture agents and an anti-DEN-NS1 antibody (Ab#D06) as a detector agent35,49. (c) Competitive IgG detection in patient blood, by modifying the DEN-NS1 detection method in (b). If a blood sample contains IgGs binding to DEN-NS1, then the DEN-NS1 detection by UB-DNA aptamers is competitively inhibited. Thus, the addition of each serotype DEN-NS1 and UB-DNA aptamer to the blood sample, followed by mixing with the detector antibody, enables the detection and quantification of IgGs specific to each DEN-NS1 serotype. (d) Typical conventional method for IgG detection, using the antigen (NS1)-coated plates to capture anti-NS1 IgGs in a blood sample.

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