Table 1 Sequences of oligonucleotide primers used for the amplification of GAPDH and bacteria Streptococcus mutans and Streptococcus sobrinus.
Target gene | Sequence | Fragment |
|---|---|---|
GAPDH | F 5ʹ TGT TCC AGT ATG ATT CCA CC 3ʹ850 bp | 850 bp |
R 5ʹ TCC ACC ACC CTG TTG CTG 3ʹ | ||
S. mutans | F 5ʹ ACT ACA CTT TCG GGT GGC TTG G 3ʹ | 517 bp |
R 5ʹ CAG TAT AAG CGC CAG TTT CAT C 3ʹ | ||
S. sobrinus | F 5ʹ GAT AAC TAC CTG ACA GCT GAC T 3ʹ | 712 bp |
R 5ʹ AAG CTG CCT TAA GGT AAT CAC T C 3ʹ |
