Figure 5
From: Amine modification of calcium phosphate by low-pressure plasma for bone regeneration

Osteogenic differentiation assay for rat BMSCs on untreated and plasma-treated β-TCP disks. (A) Schematic diagram of the osteogenic differentiation assay (drawn by using Microsoft PowerPoint 2016). (B) Macro photos of ALP-stained rat BMSCs sub-cultured on untreated (top) and plasma-treated (bottom) dense β-TCP disks. The black in the image represents the ALP stain. (C) The ALP activity values, as defined in the section Materials and methods, for untreated and plasma-treated β-TCP disks. The data here are expressed as mean ± SD (n = 3). *p < 0.05. T-test.