Figure 4 | Scientific Reports

Figure 4

From: Macrothrombocytopenia of Takenouchi-Kosaki syndrome is ameliorated by CDC42 specific- and lipidation inhibitors in MEG-01 cells

Figure 4

Y64C impaired the extension of MK protrusions. (a) To assess protrusions, the periphery (P) and area (A) of each cell were measured using software to calculate the roundness index (RI). The RI is a quantitative value that is proportional to the structural complexity of the cells regardless of their cytoplasmic mass. (b) Representative images of YFP-tagged Cdc42- or YFP-expressing MEG-01 cells cultured for 7 days in the presence of VPA. Scale bar: 10 μm (c) RI index of cells cultured for 7 days in the presence of VPA. Data are the mean ± SD (WT, n = 114, M, n = 130, CA, n = 165, YFP, n = 68) of three independent experiments. Significance was determined by the Tukey–Kramer multiple comparison test, **P < 0.01.

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