Figure 5 | Scientific Reports

Figure 5

From: The gene repertoire of the main cysteine protease of Trypanosoma cruzi, cruzipain, reveals four sub-types with distinct active sites

Figure 5

Expression profile of cruzipains in CL Brener. (A) Heatmap representing the expression values (log2RPKM) of 11 sequences coding cruzipains belonging to the two clusters in different stages of the life cycle of T. cruzi (Epi, epimastigotes; Tryp, trypomastigotes; Ama, amastigotes) in CL Brener Esmeraldo-like (EL) and non-Esmeraldo-like (NEL) haplotypes. Rows represent each sequence, and columns represent three library replicates derived from epimastigotes and intracellular amastigotes and two library replicates derived from trypomastigotes. The expression values are represented in a color grade scale, where red represents higher expression levels, and navy blue represents lower expression levels. The sequences were clustered according to their expression values. The T. cruzi life stages and cruzipain families are represented in different colors in the x and y-axis, respectively. (B) Boxplot representing the log2RPKM expression in Family I and Family II cruzipains, in the amastigote (Ama), epimastigote (Epi), and trypomastigote (Tryp) stages. (C) Z test statistics and adjusted P values (Benjamini-Hochberg) for each pair of comparisons of the Family I and Family II cruzipain gene expressions. *P values < 0.05.

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