Figure 3 | Scientific Reports

Figure 3

From: Gamma irradiation exposure for collapsed cell junctions and reduced angiogenesis of 3-D in vitro blood vessels

Figure 3

Immunofluorescence images and qRT-PCR for junction proteins (VE-cadherin and Claudin 5) showed delocalized and reduced expression after irradiation. Immunofluorescence images showed (a) delocalized VE-cadherin and (c) reduced Claudin 5 expression on the hMVECs. In addition, the relative gene expressions of (b) VE-cadherin and (d) Claudin 5 after the gamma irradiation exposure corresponded to the immunofluorescence images (scale bar: 50 µm; *p < 0.05; **p < 0.01; ***p < 0.001, n = 2–3). Statistical comparison were calculated with two-tailed t-tests using Prism (7.0a, GraphPad software, www.graphpad.com). Images and graphs were created with Adobe illustrator (CC 2019 23.0.1, www.adobe.com).

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