Figure 4 | Scientific Reports

Figure 4

From: Gamma irradiation exposure for collapsed cell junctions and reduced angiogenesis of 3-D in vitro blood vessels

Figure 4

Representative fluorescence images and calculated permeability values of endothelial barriers after irradiation. (a) The tight hMVECs’ monolayer showed a sharp decrease in the intensity (\(\Delta {\varvec{C}}\)) at the interface between the hMVECs and the remodeled ECM. In addition, the intensity gradient \(\left( {\frac{{\partial {\varvec{C}}}}{{\partial {\varvec{x}}}}} \right)\) in the ECM was measured in the normalized intensity profile with ImageJ (1.52q, NIH, www.imagej.nih.gov/ij). These intensity values vary according to the barrier permeability. (b) The permeability values of the hMVECs’ monolayer after 0, 4, and 8 Gy of gamma irradiation treatment (scale bar: 100 and 200 µm; *p < 0.05; **p < 0.01; ***p < 0.001, n = 4). Statistical comparison were calculated with two-tailed t-tests using Prism (7.0a, GraphPad software, www.graphpad.com). Images and graphs were created with Adobe illustrator (CC 2019 23.0.1, www.adobe.com).

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