Figure 5
From: SSC-ILD mouse model induced by osmotic minipump delivered bleomycin: effect of Nintedanib
Histomorphometric analysis of skin fibrosis. Evaluation of the antifibrotic activity of nintedanib on bleomycin-induced skin fibrosis through osmotic minipumps at day 28. Skin microphotographs of Saline (a, d, g), BLM (b, e, h) and BLM + NINT (c, f, i) groups. MT stained images (a, b, c—× 5) showing differences in hypodermis thickness between control and treated animals. Immunofluorescent staining for Collagen Type I (d, e, f—× 15) showing different levels of expression in control and treated animals. H&E staining (g, h, i—× 30) showing different inflammatory infiltrates in control and treated animals. Measures of dermis thickness (j), hypodermis thickness (k) and evaluation of the inflammation score (i) were carried out at five randomly selected fields from a sample of each animal. Data are shown as mean values ± S.E.M. for 8 mice per group. Changes were compared to the BLM group using one-way ANOVA followed by Dunnett’s test (**P < 0.01; ***P < 0.001). The percentages on the BLM + NINT bars represent the inhibitory effect of the treatment compared to BLM.
