Figure 2

Identification of PrPSc in white-tailed deer gestational tissues and fluids by western blot and PMCA. Western blot analysis of representative gestational tissue samples prior (A) and after (B) PK treatment. (C) PMCA results from the same representative samples depicted in (A) and (B) after three PMCA rounds. Numbers at the right of each panel represent molecular weight markers (in KDa). “PrPC” denotes brain extracts from Tg1536 mice not treated with PK and used as additional molecular weight markers.