Figure 6
In vivo safety of CaD23 in various concentrations [0.03% (or 300 μg/ml), 0.05% (or 500 μg/ml), and 0.1% (1 mg/ml)] and phosphate buffer solution (PBS) assessed in a murine corneal epithelial wound healing model (n = 4 mice/treatment group). (A) Representative slit-lamp images showing the daily progress of corneal wound healing of each treatment group. The green color-stained area depicts the corneal epithelial defect. Complete corneal re-epithelialization was observed in all treatment groups, except CaD23 0.1% group, by day 3. The images were analyzed using ImageJ software (https://imagej.nih.gov/ij/)67. (B) Graphical summary of the progress of corneal re-epithelialization of each treatment group over 3 days. The corneal epithelial wound size at various time points is calculated based on the original 100% wound size at baseline. Data is presented as mean ± standard deviation.
