Table 1 Summary of the native and synthetic host defense peptide (HDP) sequences.

Types	Peptides	Peptide sequence	Number of AA	Molecular weight, g/mol	Net charge	Hydrophobicity, <H>	Hydrophobic moment, <µH>
Native	HBD1	DHYNCVSSGG QCLYSACPIF TKIQGTCYRG KAKCCK	36	3934.6	+ 4	0.431	0.348
	HBD2	GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P	41	4334.2	+ 6	0.489	0.246
	HBD3	GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK	45	5161.2	+ 11	0.228	0.097
	LL37	LLGDFFRKSK EKIGKEFKRI VQRIKDFLRN LVPRTES	37	4493.3	+ 6	0.201	0.521
Synthetic single HDPs	Ca12	KRIVQRIKDF LR	12	1571.9	+ 4	0.193	0.782
	BD2^a	KCCKKP	6	705.9	+ 3	–	–
	BD3	RGRKCCRRKK	10	1290.6	+ 7	− 0.393	0.300
1st generation HyHDPs	DD12	RGKAKCCKGT KCCKKP	16	1739.2	+ 7	0.031	0.144
	DD13	RGKAKCCKRG RKCCRRKK	18	2165.7	+ 11	− 0.251	0.200
	DD32	RGRKCCRRKK KCCKKP	16	1978.5	+ 10	− 0.194	0.201
	CaD1	KRIVQRIKDF LRRGKAK	17	2112.6	+ 7	− 0.022	0.383
	CaD2	KRIVQRIKDF LRKCCKKP	18	2259.8	+ 7	0.174	0.445
	CaD3	RIKDFLRNGR KCCRRKK	17	2177.7	+ 8	− 0.118	0.234
2nd generation HyHDPs	CaD21	KRIVQRIKDF LRKACKKP	18	2227.8	+ 7	0.106	0.443
	CaD22	KRIVQRIKDW LRKCCKKP	18	2298.9	+ 7	0.200	0.456
	CaD23	KRIVQRIKDW LRKLCKKW	18	2398.0	+ 7	0.294	0.456

Calculation of the physicochemical properties of the peptides was performed using PepCalc (https://pepcalc.com/) and HeliQuest (https://heliquest.ipmc.cnrs.fr/cgi-bin/ComputParams.py).
HBD: human beta-defensin; AA: amino acids; HyHDPs: hybrid host defense peptides; C: cysteine; D: aspartic acid; E: glutamic acid; F: phenylalanine; G: glycine; I: isoleucine; K: lysine; L: leucine; N: aspargine; P: proline; Q: glutamine; R: arginine; S: serine; T: theorine; V: valine; W: tryptophan.
^aThe sequence is too short for calculation of hydrophobicity and hydrophobic moment.

Quick links

Search