Figure 1

Variations in RT-qPCR Cq values of eight tomato genes/alleles [viz. actin (ACT), adenine-phosphoribosyl-transferase 1 (APT), β-2-tubulin (TUB2), elongation factor 1-alpha (EF1α), the Arabidopsis thaliana expressed protein (EXP), TIP41-interacting protein (TIP41), phytoene desaturase (PDS), and ubiquitin (UBI3 = UBQ)] employed in the assays involving the pathosystem tomato ‘Hawaii 7996’ and compatible/virulent and incompatible/avirulent Ralstonia solanacearum isolates at different sample collection times. Box charts of Cq for each reference gene in samples of tomato ‘Hawaii 7996’ infected by R. solanacearum under different experimental conditions; (Panel A) Interactions of ‘Hawaii 7996’ with two compatible/virulent isolates compared to resistance at 24 HPI (hours post-inoculation) and 96 HPI and mock-inoculated (0 HPI). (Panel B) Interaction of ‘Hawaii 7996’ with an incompatible/avirulent isolate at 24 and 96 HPI compared to control mock-inoculated (0 HPI). The horizontal lines and small squares in the box plot represent median values and mean values respectively, surrounded by lower and upper boxes indicating the first and third quartile. Vertical lines indicate the value ranges.