Table 2 Analyses of expression stability of eight candidate reference genes for expression normalization involving the pathosystem tomato ‘Hawaii 7996’ and virulent/compatible and avirulent/incompatible Ralstonia solanacearum isolates at different sampling collection times.

Gene ranking	geNorm		NormFinder		BestKeeper
Gene ranking	Gene #01^a/#02	M^b #01^a/#02	Gene #01^a/#02	Stability^c #01^a/#02	Gene #01^a/#02	Stability^d #01^a/#02
1	PDS/TIP41	0.205/0.032	TIP41/TIP41	0.004/0.004	UBI3 /UBI3	0.734/0.482
2	TIP41/EF1α	0.205/0.034	EXP/ACT	0.004/0.004	PDS/TIP41	0.820/0.508
3	UBI3/ACT	0.205/0.040	ACT/EXP	0.005/0.005	EXP/PDS	0.820/0.525
4	EXP/PDS	0.221/0.119	UBI3/PDS	0.008/0.009	ACT/ACT	0.853/0.531
5	ACT/EXP	0.245/0.154	PDS/UBI3	0.011/0.011	TIP41/EXP	0.862/0.584
6	TUB2/UBI3	0.291/0.176	TUB2/EF1α	0.013/0.019	TUB2/APT	0.932/0.666
7	APT/TUB2	0.392/0.292	APT/APT	0.016/0.021	APT/TUB2	0.942/0.807
8	EF1α/APT	0.494/0.456	EF1α/TUB2	0.022/0.021	EF1α /EF1α	1.974/1.445
BCTG	PDS/TIP41 and TIP41/EF1α	…	TIP41/EXP and TIP41/ACT	…	…	…

BCTG best combination of two genes.
^aGlobal Interaction #01 and Global Incompatible Interaction #02, as described in Table 1.
^bM = average expression stability, calculated using the algorithm geNorm.
^cStability = gene expression stability calculated by intragroup and intergroup variation using the algorithm NormFinder.
^dStability = standard deviation (std dev [± CP]) calculated using the algorithm BestKeeper. Significant values are the ones displaying values < 1.0. All genes showed significant p value (= 0.001) using Pearson's correlation test.

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