Table 3 Supporting information on the selected primer pairs and amplification profiles of the candidate reference genes for reverse transcription-quantitative PCR (RT-qPCR) normalization involving the pathosystem tomato ‘Hawaii 7996’ and compatible/virulent and incompatible/avirulent Ralstonia solanacearum isolates.

From: Stability analysis of reference genes for RT-qPCR assays involving compatible and incompatible Ralstonia solanacearum-tomato ‘Hawaii 7996’ interactions

Gene symbol

Gene name

Sequence 5′–3′ (F and R)

PCR amplicon length (bp)

Amplification efficiency (%) ± SD

References

ACT

Actin

CGGTGACCACTTTCCGATCT

62

103.0 ± 0.0196

25

TCCTCACCGTCAGCCATTTT

APT

Adenine-phosphoribosil-transferase1

GAACAGACAAGATTGAGATGCATGTA

60

94.3 ± 0.0222

25

CCACGAGGGCACGTTCA

TUB2

β-2-tubulin

TTGGTTTTGCACCACTGACTTC

84

95.5 ± 0.0232

25

AAGCTCTGGCACTGTCAAAGC

EF1α

Elongation factor 1-α

GATTGACAGACGTTCTGGTAAGGA

67

86.0 ± 0.0204

25

ACCGGCATCACCATTCTTCA

EXP

Expressed sequence

GCTAAGAACGCTGGACCTAATG

183

94.4 ± 0.0250

26

TGGGTGTGCCTTTCTGAATG

PDS

Phytoene Desaturase

GCCGATTGTGGAACATATTGAGTC

91

91.4 ± 0.0193

28

GACACTTCCATCCTCATTCAGCTC

TIP41

TIP41-interacting protein

ATGGAGTTTTTGAGTCTTCTGC

235

92.3 ± 0.0162

26

GCTGCGTTTCTGGCTTAGG

UBI3

Ubiquitin 3

AGAAGAAGACCTACACCAAGCC

119

95.1 ± 0.0280

28

TCCCAAGGGTTGTCACATACATC

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