Figure 6

Deep mutational scanning of PAI-1 provides insight into the mutational landscape of PAI-1’s RCL. Relative log₂-fold change scores of missense mutations in PAI-1’s RCL (amino acids 331–350) following selection by ability to inhibit uPA³⁹. Amino acids below the x-axis indicate depletion (log₂-fold change < 0), while those above the x-axis indicate enrichment (log₂-fold change > 0). Letter height corresponds to the relative log₂-fold change. Residues are color coded by properties: acidic residues in red, basic residues in blue, polar amino acids in green, non-polar amino acids in orange, and Pro in purple³⁹. The P1 and P1′ positions (Met³⁴⁶ and Arg³⁴⁷ in WT) are also indicated. This figure was generated using Adobe Illustrator 2021 version 25.2.3 and Seq2Logo 2.0 (http://www.cbs.dtu.dk/biotools/Seq2Logo/).