Figure 5 | Scientific Reports

Figure 5

From: Deleting a UBE3A substrate rescues impaired hippocampal physiology and learning in Angelman syndrome mice

Figure 5

Knockdown of UBE3A leads to an activity dependent increase in dendritic spine formation that requires Ephexin5. (A) Hippocampal neuronal cultures were transfected at DIV 10 with plasmids containing GFP, an shRNA hairpin, and hM4Di, and treated with CNO or vehicle from DIV 11–14. Representative neurons at DIV 14 are shown, with white arrows indicating the soma of the transfected cells. Cells were stained for UBE3A (red) and GFP (green). Nuclei were labeled with Hoescht. (B) For each treatment group, spine density is shown as spines per micron across the entire transfected neuron. Data are presented as mean ± SEM (n = 3 for all genotypes). *p < 0.05 (two-way ANOVA) within genotype with post-hoc Sidak’s multiple comparisons test. Exact p values are reported Supplementary Table 2 and Supplementary Table 3.

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