Figure 1
From: Toxicity of amantadine hydrochloride on cultured bovine cornea endothelial cells
Effect of amantadine HCl on cell growth of bovine cornea endothelial cells. (A) BCE C/D-1b cells were treated with various doses of amantadine HCl (0–2000 μM) for 24 h. Cell morphology was monitored by phase-contrast microscopy. The red arrow indicates dead cells that became detached and floated after treatment. Assays were carried out in triplicates, and the results are representative of three independent experiments. (B) BCE C/D-1b cells were treated with various concentrations of amantadine HCl (0–2000 μM) for 1–7 days. Cell viability was examined by MTT assay. The data are presented as means ± SD of three independent experiments. Statistical significance was represented as follows: *p < 0.05 or **p < 0.01 vs. untreated control.
