Figure 5
From: Toxicity of amantadine hydrochloride on cultured bovine cornea endothelial cells
Effect of amantadine HCl on cell proliferation in bovine cornea endothelial cells. BCE C/D-1b cells were treated with different concentrations of amantadine HCl (0–2000 μM) for 24 h. (A) The DNA synthesis was examined by EdU incorporation assay. Overlay of the histograms of untreated cells (green) and cells treated with amantadine HCl (red). (B) The cell proliferation index was quantified and the statistical significance was represented as follows: **p < 0.01 vs. untreated control. BCE C/D-1b cells were labeled with 1 μΜ CFSE for 10 min and then treated with different concentrations of amantadine HCl (0–2000 μM) for seven days. (C) The results are representative of three independent experiments. The CFSE histograms of amantadine HCl treated cells (red) were overlaid with untreated cells (green). (D) Statistical analysis was carried out from three independent experiments. Data are presented as means ± SD and the statistical significance was represented as follows: *p < 0.05, **p < 0.01 and ***p < 0.001 vs. untreated control.
