Figure 6

Effect of amantadine HCl on endothelial permeability in bovine cornea endothelial cells. BCE C/D-1b cells were seeded in the upper chamber of 0.4 μm transwell inserts and treated with different concentrations of amantadine HCl (0–2000 μM) or DTX (10 and 100 nM) for 24 h. Afterward, FITC-dextran (1 mg/mL) was added into the upper chamber, then the lower chamber media was collected after 0, 20, 40 or 60 min, and fluorescent intensity was measured (ex: 485 nm; em: 535 nm) using a fluorescence plate reader. The absolute permeability was presented as means ± SD of three independent experiments. The statistical significance was represented as follows: *p < 0.05, **p < 0.01 and ***p < 0.001 vs. untreated control.