Figure 3 | Scientific Reports

Figure 3

From: The role of autophagy in the process of osseointegration around titanium implants with micro-nano topography promoted by osteoimmunity

Figure 3

Comparison of the proliferation, differentiation and mineralization levels of osteoblasts stimulated with or without macrophage medium supernatant. (A) CCK-8 was used to detect the proliferation activity of MC3T3-E1 cells at 1, 3, 5, 7 days; (B) western blot showed the expression of osteogenic related proteins runx2 and collagen I at 7 days; (C) real-time PCR showed the expression of osteogenic marker genes runx2 and collagen I at 3 and 7 days; (D) alizarin red showed the mineralization of MC3T3-E1 cells [OM osteogenic induction medium, CM conditional medium (osteogenic induction medium and the supernatant of RAW264.7 cells’ culture medium). *p < 0.05; **p < 0.01; ***p < 0.001].

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