Figure 4 | Scientific Reports

Figure 4

From: The role of autophagy in the process of osseointegration around titanium implants with micro-nano topography promoted by osteoimmunity

Figure 4

Detection of autophagy level of MC3T3-E1 cells under OM and CM culture conditions with or without 3-MA. (A) MDC autophagy body staining (Autophagosomes were shown in green); (B) real-time quantitative PCR showed the expression of autophagy related genes LC3 and P62 at day 3 and 7. (C) Western blot showed the expression of LC3II/[OM osteogenic induction medium, OM/3-MA adding 5 mM autophagy inhibitor 3-MA to osteogenic induction medium, CM conditional medium (osteogenic induction medium and the supernatant of RAW264.7 cells’ culture medium), CM/3-MA adding 5 mM autophagy inhibitor 3-MA to the conditioned medium. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001].

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