Figure 4

Pretreatment with a gradient concentration of WZYZ could restore the proliferation activity of rat testicular germ cells induced by acute THS. The expression of Ki67 in germ cells was detected by immunohistochemistry. A representative image shows Ki67 positive germ cells in the brown area (red arrow). Bar = 100 μM. (a) Control group; (b) model group; (c) low-dose WZYZ group; (d) middle-dose WZYZ group; (e) High-dose WZYZ group; (f) bar chart describing the percentage of Ki67 positive area. The data showed an average ± SEM (n = 3 rat per group). **Compared with the control group, P < 0.01; ##compared with the model group, P < 0.01.