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Figure 1

From: Inflammatory signature in acute-on-chronic liver failure includes increased expression of granulocyte genes ELANE, MPO and CD177

Figure 1

Overall study design and neutrophil enrichment. (A) Polymorphonuclear neutrophils (PMN) were enriched from the peripheral whole blood of participants. The isolated PMN were assessed for neutrophil enrichment by CD16 surface staining and analyzed by flow cytometry. RNA were extracted from enriched neutrophils and subjected to microarray analysis followed by quantitative RT-PCR validation of top differentially expressed genes. (B, C) Flow cytometry based evaluation of the enriched PMN population based on CD16 and CD66b surface staining of representative ACLF (B) and CLD (C) samples.

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