Figure 4 | Scientific Reports

Figure 4

From: Violet bioluminescent Polycirrus sp. (Annelida: Terebelliformia) discovered in the shallow coastal waters of the Noto Peninsula in Japan

Figure 4

Comparison of the ethanolic extract of Polycirrus sp. ISK with CTZ, CTMD, and CTM. (A) UPLC analysis of (a) the extract, (b) authentic CTZ, (c) authentic CTMD, and (d) authentic CTM using a multiwavelength detector. The black solid line indicates detection at 333 nm, and the blue solid line indicates detection at 435 nm. The compound between the red vertical dashed lines was collected for MS/MS analysis. (B) Absorption spectra of the compound from the extract, CTZ, CTMD, and CTM obtained at retention times of (a) 9.65, (b) 10.89, (c) 9.47, and (d) 9.27 shown in (A). CTZ coelenterazine, CTMD coelenteramide, CTM coelenteramine. These chemical structures are shown in Supplementary Fig. S5.

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