Table 2 Limit of Detection (LoD) of our assay evaluated using Research Reagent for SARS-CoV-2 RNA (NIBSC code 19/304) from the National Institute for Biological Standard and Control.

From: A new qualitative RT-PCR assay detecting SARS-CoV-2

Sample no

RIDAGENE SARS-CoV-2 test (single target ORF1 ab gene)

Real-Time Fluorescent RT-PCR Kit for Detecting SARS-CoV-2-BGI (single target: E-gene)

MOLgen SARS CoV-2 Real Time RT PCR Kit (three targets, E, N, RdRp gene)

Allplex SARS-CoV-2 (four targets: E, N, RdRp/S gene)

34

Positive

Positive

Positive

Positive

1

Positive

Negative

Negative

Positive

1

Positive

Negative

Positive

Positive

1

Positive

Positive

Positive

Negative

1

Positive

Negative

Positive

Negative

1

Positive

Negative

Positive

Positive

1

Positive

Positive

Negative

Positive