Figure 3

The Ripk3 expression in tissues and inflammatory cells and CD4 + T cell profiles in Ripk3^−/− SKG mice. (A) Quantitative RT-PCR to detect the expression of Ripk3 in whole synovial tissues of healthy and inflamed joints (n = 4 each). (B) Quantitative RT-PCR to detect the Ripk3 mRNA expression in CD4⁺ T cells, CD11b⁺Ly6G⁺ neutrophils, CD11b⁺Ly6G^-Ly6C^high inflammatory monocytes and CD45^-CD31^- Podoplanin⁺ FLS in inflamed joints (n = 4 each). (C) The IL-1β concentration of culture supernatant of BMDCs at 16 h after LPS stimulation (n = 4 each) (D) Quantitative RT-PCR to detect the expression of Ripk3 in the indicated organs of BALB/c mice housed under SPF or GF conditions (n = 4 each). (E,F) Flow cytometry of IL-17A⁺, GM-CSF⁺, IFN-γ⁺ (n = 3 each) and Foxp3⁺ cells (n = 4 each) in CD4⁺ T cells in Peyer’s patches of WT and Ripk3^−/− SKG mice. The vertical bars denote the SD in panel (A–C,F) and SEM in panel (D). *P < 0.05. Data are pooled from two independent experiments in panels (A–D,F).