Figure 6 | Scientific Reports

Figure 6

From: The macronuclear genome of the Antarctic psychrophilic marine ciliate Euplotes focardii reveals new insights on molecular cold adaptation

Figure 6

Hsp70 transcript abundance in stressed E. focardii cells normalized to the abundance in control cells. mRNA levels were determined by qPCR of the Hsp70 isoforms (reported as protein assembly code) using the comparative threshold method. Mean ± SD (n = 4). Total RNA was purified from cells grown at their standard temperature of 4° C (i.e., not shocked), from cells which were exposed to 18 °C (orange) via a heat ramp (see "Materials and methods" section), or from cells exposed to 100 µM of hydrogen peroxide (blue); control and experimental treatments were for 30 min. Data are reported as the mean of three experiments.

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