Figure 3

Time evolution of strain around the equatorial plane along M-L, A-P and radial (‘r’) directions. (A) The dorsal image of the embryo at 8, 10, 10.5 and 11 hpf of zebrafish development. Strain is calculated along the embryo equator (white band). Scale bar—100 µm. (B–D) Kymograms of strain along (B) M-L, (C) A-P, and (D) radial, ‘r’, directions and from 7.5 to 11.5 hpf (x-axis). The Y-axis measured strains around the circumference (white band in (A) of the embryo) from − 180° (Ventral side) to 0° (Dorsal side) to 180° (ventral side) in the anticlockwise directions. Dashed lines correspond to stages in (A). Expansion state in both side of the dorsal line at 10:30 hpf, is marked with “*” symbol.