Figure 1 | Scientific Reports

Figure 1

From: Cyp2c44 epoxygenase-derived epoxyeicosatrienoic acids in vascular smooth muscle cells elicit vasoconstriction of the murine ophthalmic artery

Figure 1

Expression and localization of Cyp2c44 in the mouse ophthalmic artery. Representative photomicrographs depicting the expression of Cyp2c44 in sagittal cryosections of the ophthalmic artery from (a) wild type (WT) and (b) Cyp2c44−/−mice using immunofluorescence staining. The magnified section of the artery shows the localization of this enzyme (in red) predominantly in the vascular smooth muscle cells stained with α-SMA (in cyan). Endothelial cells were stained green with CD31 and cell nuclei were stained blue with DAPI. (c) Western blot analysis of Cyp2c44 in the WT artery compared to Cyp2c44−/− and sEH−/− arterial samples. Cyp2c44 protein level was normalized to β-actin. Scale bars indicate 50 µm and 20 µm for lower and higher power magnification, respectively. Samples were pooled from n = 5 mice/ WT and n = 6 mice/ Cyp2c44−/− and sEH−/− each, per Western blot analysis. The Cyp2c44 and β-actin bands were cropped from full gels (Supplementary Fig. S1 online).

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