Figure 1
From: TDP-43 regulates GAD1 mRNA splicing and GABA signaling in Drosophila CNS
TBPH modulates GAD1 protein expression by regulating its pre-mRNA 5’-UTR splicing. (a) Scheme of Gad1 genomic locus and transcripts in Drosophila. (b) qRT-PCR of Gad1 pre-mRNA left panel and mRNA right panel on adult heads of w1118, tbphΔ23/Δ23 and tbphΔ142/Δ142. n = 3; *p < 0.05 and **p < 0.01 calculated by one-way ANOVA. (c) Western blot analysis for GAD1 minigene expression in S2 cells (anti-EGFP, anti-TBPH, and anti-Tubulin). Full-length gels in Supplementary Information. Sample 1: S2 cells co-transfected with GAD1 minigene, Actin-GAL4 and Luciferase-RNAi. Sample 2: S2 cells co-transfected with GAD1 minigene, Actin-GAL4, and TBPH-RNAi. Sample 3: S2 cells only. In the graph the quantification of EGFP and TBPH expression weighted on Tubulin of Luciferase-RNAi (black column) compared with TBPH-RNAi (white columns), in light gray S2 cells only as negative control. ****p < 0.0001; calculated by one-way ANOVA. In the scheme GAD1 minigene: the 5’ UTR region of Gad1 spanning from exon1 to exon2 in frame with a reporter EGFP has been cloned in pUAST attB plasmid. n = 2, Error bars SEM.
