Table 1 Sequences of primers used for quantitative RT-PCR in mice.
Gene name | Primers (forward and reverse 5′ to 3′) |
|---|---|
ICAM-1 | F: 5′-TTCACACTGAATGCCAGCTC-3′ R: 5′-GTCTGCTGAGACCCCTCTTG-3′ |
VCAM-1 | F: 5′-CCCAGGTGGAGGTCTACTCA-3′ R: 5′-CAGGATTTTGGGAGCTGGTA-3′ |
P-selectin | F: 5′-GTCCACGGAGAGTTTGGTGT-3′ R: 5′-AAGTGGTGTTCGGACCAAAG-3′ |
E-selectin | F: 5′-AGCTACCCATGGAACACGAC-3′ R: 5′-ACGCAAGTTCTCCAGCTGTT-3′ |
TLR2 | F: 5′-CAGCTGGAGAACTCTGACCC-3′ R: 5′-CAAAGAGCCTGAAGTGGGAG-3′ |
TLR4 | F: 5′-CAACATCATCCAGGAAGGC-3′ R: 5′-GAAGGCGATACAATTCCACC-3′ |
NF-κB | F: 5′-ATGGCAGACGATGATCCCTAC-3′ R: 5′-TGTTGACAGTGGTATTTCTGGTG-3′ |
