Figure 4
Oral CuATSM treatment increases soluble SOD1 levels and activity in the lumbar spinal cord of SOD1G93A mice. (a) The relative levels of SOD1 protein were determined via western blot in (b). PBS-soluble fractions obtained from the lumbar spinal cord of vehicle- and CuATSM-treated mice. Quantification of relative SOD1 levels were normalised to total protein loading for each sample. (c) PBS-soluble homogenate from the lumbar spinal cord of CuATSM- and vehicle-treated mice were separated on a native 8% gel and SOD1 activity determined by in-gel zymography. Equal total protein amount across samples were determined by Coomassie signal. (d) Quantification of relative SOD1 normalised to total protein. Data shown are means ± SEM (n = 6/treatment). Data are from two independent experiments. Student’s t test was used to compare relative differences between CuATSM- and vehicle-treated mice SOD1G93A mice. Full-length blots and gels are presented in Supplementary Figures 2 and 3, respectively.
