Table 2 Concordance analysis per embryo.

From: High concordance in preimplantation genetic testing for aneuploidy between automatic identification via Ion S5 and manual identification via Miseq

 

Ion S5

Miseq

Number of embryos tested

107

107

Number of embryos screened by NGS

107

107

Number of embryos with conclusive result a

104

104

Number of embryos with inconclusive result

3

3

Number of embryos with aneuploid calling b

54

55

Ploidy conclusion

Euploid

50 (48.0%)

49 (47.1%)

Low-rate mosaic (20–50% aneuploidy)

6 (5.8%)

11 (10.6%)

High-rate mosaic (50–80% aneuploidy)

8 (7.7%)

9 (8.7%)

Aneuploid

40 (38.5%)

35 (33.6%)

 Single-chromosome aneuploidy

28

24

 Double-chromosome aneuploidy

5

3

 Multiple aneuploidy c

7

8

Number of concordant embryos d

103

 

Number of non-concordant embryos e

1

 

Concordance per embryo

99.04%

 
  1. aIt excludes embryos with chaotic patterns that the ploidy could not be interpreted.
  2. bIt includes embryos with mosaicism and aneuploidy.
  3. cIt includes embryos with more than two aneuploid chromosome pairs.
  4. dIt includes embryos identified as euploid or mosaic/aneuploid on the both two NGS systems.
  5. eEmbryos identified as euploid on only one NGS system, and as mosaic or aneuploid on the other system.
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