Table 2 Diversity estimation of the 16S rDNA gene libraries of water from the V-troughs on different tiers from sequencing analysis.

From: Determination of bacterial abundance and communities in the nipple drinking system of cascading cage layer houses

Group

Effective reads

Average length

OTU

ACE

Chao

Shannon

V1

76,443.50 ± 9142.28

434.92 ± 54.42

1027.67 ± 138.60

1483.03 ± 201.74

1483.46 ± 185.88

6.34 ± 0.61

V3

71,255.67 ± 5433.72

427.08 ± 66.69

1043.33 ± 70.51

1454.19 ± 78.79

1301.61 ± 81.52

6.08 ± 0.63

V5

70,947.50 ± 3835.73

446.83 ± 33.06

1049.50 ± 139.61

1525.86 ± 183.50

1482.53 ± 171.49

6.47 ± 0.69

V7

72,403.83 ± 3436.28

444.92 ± 40.52

984.00 ± 91.49

1398.63 ± 173.29

1371.20 ± 179.35

6.09 ± 0.54

  1. Operational taxonomic units (OTUs) were defined at 3% dissimilarity. Richness estimators (ACE and Chao) and diversity indices (Shannon) were calculated; V1, V3, V5, and V7 represent water samples from V-trough on the 1st, 3rd, 5th and 7th tier, respectively.