Figure 1
From: Mechanism of the fungal-like particles in the inhibition of adipogenesis in 3T3-L1 adipocytes
LCB inhibits adipogenesis in a dose-dependent manner. Preadipocytes (A), fully differentiated adipocytes (B), differentiating adipocytes treated with uncoated beads (1:150 cells:UC) (C), 100 ng/mL LPS (D), and an increasing amount of LCB (1:50, 1:100, and 1:150 cells:LCB ratios in E–G) were stained with Oil Red O dye, which binds to triglycerides in the cells. LPS, a known adipogenic inhibitor, and LCB were added to the cells from the beginning of differentiation and maintained throughout adipogenesis. (H) shows the spectrophotometric absorbance at 520 nm for the Oil Red O dye that binds to triglycerides in the cells. The results are presented as the relative lipid accumulation percentages in respect to the PA set. (I) shows the quantitative measurement of the total triglycerides in the cells using the total triglyceride assay. PA, UT, and UC are abbreviated for preadipocytes, untreated, and uncoated beads, respectively.
