Figure 1
taken from the independent mouse samples (day 3, 56, 112, 168: n = 10 in each group, 5 males and 5 females). (H) Quantitative analysis of single-colored areas occupying JE (day 3, 56, 112, 168: n = 10 mice, 30 clones in each group, 5 males and 5 females). The differences between the four groups using one-way analysis of variance, followed by Tukey’s honestly significant difference. Error bars show s.e.m. *P < 0.0001. Scale bars represent 50 μm. OGE Oral gingival epithelium, JE Junctional epithelium.
Single-color areas replaced all the constituent cells of the junctional epithelium (JE) over time. (A) The strategy to show the presence of stem cells. The upper panel represents a schema of the construct of the Rosa26-rainbow system. The lower panels are a schema of the multicolor lineage tracing method to demonstrate the presence of the stem cells. (B) Time course showing the experimental design. (C) Representative hematoxylin and eosin (H&E) stained image of the first molar and its surrounding tissue. (D, G) Representative fluorescence images of the transverse sections of the JE in the Rosa26CreERT2/rbw mice on day 3 (D), 56 (E), 112 (F), 168 (G) after tamoxifen administration. The white dotted lines indicate the region of the JE. Representative images were
