Table 1 Plasmids and ratios used to produce lentiviral vectors in this study.
Generation | Name | Addgene number | Plasmid | Ratio | Reference |
|---|---|---|---|---|---|
2nd | 2A | 12,254 | Transfer plasmid: pWPI | 5 | Trono Lab Constitutive Lentiviral Plasmids (unpublished) |
8455 | Packaging plasmid: pCMV-dR8.2 dvpr | 2 | Stewart et al. (2003) | ||
12,259 | Envelope plasmid: pMD2.G | 1 | Trono Lab Constitutive Lentiviral Plasmids (unpublished) | ||
2nd | 2B | 12,252 | Transfer plasmid: pRRLSIN. cPPT. PGK-GFP. WPRE | 4 | Trono Lab Constitutive Lentiviral Plasmids (unpublished) |
12,260 | Packaging plasmid: psPAX2 | 3 | Trono Lab Constitutive Lentiviral Plasmids (unpublished) | ||
12,259 | Envelope plasmid: pMD2.G | 1 | Trono Lab Constitutive Lentiviral Plasmids (unpublished) | ||
3rd | 3A | 19,319 | Transfer plasmid: pLJM1-EGFP | 4 | Sancak et al. (2008) |
12,251 | Packaging plasmid: pMDLg/pRRE | 2 | Dull et al. (1998) | ||
12,253 | Packaging plasmid: pRSV-Rev | 1 | Dull et al. (1998) | ||
12,259 | Envelope plasmid: pMD2.G | 1 | Trono Lab Constitutive Lentiviral Plasmids (unpublished) | ||
3rd | 3B | 19,319 | Transfer plasmid: pLJM1-EGFP | 4 | Sancak et al. (2008) |
12,251 | Packaging plasmid: pMDLg/pRRE | 2 | Dull et al. (1998) | ||
12,253 | Packaging plasmid: pRSV-Rev | 1 | Dull et al. (1998) | ||
8454 | Envelope plasmid: pCMV-VSV-G | 1 | Stewart et al. (2003) |
